Efficient isolation of mumps virus from a community outbreak using the marmoset lymphoblastoid cell line B95a.

Molecular cloning of membrane cofactor protein (MCP; CD46) on B95a cell, an Epstein-Barr virus-transformed marmoset B cell line: B95a-MCP is susceptible to infection by the CAM, but not the Nagahata strain of the measles virus.

Measles virus (MV) infects not only human beings but also some simian species. The MV receptor on Vero cells (a cell line established from African Green monkey kidney cells) and human cells has been shown to be the membrane cofactor protein MCP/CD46, which is an inhibitor of autologous complement (C) activation. B95a, an Epstein-Barr virus (EBV)-transformed marmoset B cell line, is a simian cel...

Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells.

We have previously shown that canine signaling lymphocyte activation molecule (SLAM; also known as CD150) acts as a cellular receptor for canine distemper virus (CDV). In this study, we established Vero cells stably expressing canine SLAM (Vero.DogSLAMtag cells). Viruses were isolated in Vero.DogSLAMtag cells one day after inoculation with spleen samples from five out of seven dogs with distemp...

Adaptability to Marmoset B95a Cells Canine SLAM (CD150) and Their Distemper Virus in Vero Cells Expressing Efficient Isolation of Wild Strains of Canine

Leucine at position 383 of fusion protein is responsible for fusogenicity of wild-type mumps virus in B95a cells.

OBJECTIVE Mumps virus is isolated in Vero cells and, recently, B95a cells have been reported to be susceptible to it. Currently circulating wild-type mumps virus strains (genotypes B, G, J and L) induced cytopathic effects in both Vero and B95a cells. On the other hand, the Hoshino vaccine strain (KO3) did not induce cytopathic effects in B95a cells. In this study, differences in fusion inducib...

Isolation, quarantine, social distancing and community containment: pivotal role for old-style public health measures in the novel coronavirus (2019-nCoV) outbreak.

New coronavirus as a pandemic threatens whole world. This virus as a member of coronavirus family has new and unknown characteristics and no vaccine or treatment agent has been developed for this infectious disease. Regarding the successful experience of public health measures in SARS outbreak controlling and haltering, the infected countries have applied these proceedings widely to control new...